truCOVER® Total RNA Library Prep Kits

Clinical and biopharmaceutical labs can't afford compromise. When samples are degraded, timelines are tight, and data quality is non-negotiable, your RNA library prep must perform - every single time, for every single sample. truCOVER Total RNA Library Prep isn't incremental process improvement, it's a workflow upgrade for generating sequence-ready RNA libraries from FFPE, whole blood, and other sample types.

High-performance RNA library prep for Challenging Samples

High-Performance RNA Library Prep for Challenging Samples

Class-leading Adaptive Focused Acoustics® (AFA®) Technology drives every step, delivering strand-specific libraries with the precision and consistency your data demands.
If your lab struggles to prepare quality libraries from degraded inputs, adequately detect gene fusions, and maintain low-input performance with solid reproducibility, this is your solution.

truCOVER Total RNA Library Prep Features & Benefits:

High-fidelity representation across the transcriptome

• Superior detection of gene fusions and alternative splicing events even in challenging FFPE at lower sequencing depths, for confident results at lower sequencing cost

• Higher proportion of exonic reads captured; over 80% mapping to protein-coding regions, maximizing informative data from every flow cell

• Ultra-efficient depletion (>99%) of cytoplasmic and mitochondrial rRNA, as well as globin mRNA, supporting greater dynamic range, without sacrificing library complexity

Robust, repeatable results

• Repeatable NGS-ready RNA libraries minimize technical variation across runs and operators

• Consistent insert sizes, even for degraded RNA, supporting sequencing success from FFPE with DV200 ≥30%, or whole blood RIN ≥ 5

• Reproducible library yields across a broad input range (10–500 ng), enabling accurate gene expression analysis and other RNA-Seq applications

A faster, simpler, end-to-end workflow

• A 4-hour protocol combines and optimizes key steps to reduce workflow time by 40% versus traditional methods, enabling higher sample throughput per run

• Minimal hands-on time and fewer workflow steps, in 24- or 96-sample formats — simple enough for anyone to run, flexible enough to scale with your automation platform

• Pair with Covaris' truXTRAC® FFPE extraction kits, for a seamless, AFA-driven workflow from extraction to library prep

• Optimized, single-vendor chemistry and fewer handoffs mean less variability from degraded samples to high-quality sequencing-ready libraries

• Deplete rRNA and globin mRNA in the same 15-minute step with the optional globin depletion module

truCOVER Total RNA Library Prep Kits require Covaris Unique Dual Index (UDI) sets.
Dual indexing enables the addition of adapters needed to bind to the flow cell for sequencing and minimizes index misassignment, particularly important for low-input, FFPE, or other degraded RNA samples.

Covaris UDI Plates for truCOVER Total RNA Library Prep can process up to 96 samples, either in a single run or during multiple successive runs. Covaris offers 8 UDI set options to support flexible multiplexing needs.
Detailed index sequences and compatibility information are available in the Sample ID and UDI Reference File linked below under Protocols.

A complete AFA-powered workflow for RNA-Seq Library Prep

truCOVER Total RNA Library Prep Kit Contents

Covaris RNA UDI Set Content

The truCOVER portfolio also includes flexible solutions for DNA library preparation. Explore truCOVER® DNA Library Prep Kits for high-quality genomic workflows.

Want to learn more? Contact us to speak to a rep.