The AFA-TUBE is a specially engineered polymer vessel designed for DNA Shearing at sample volumes ranging from 10 to 20 ul with truSHEAR. It can be used to improve turnaround time and throughput on automation platforms in NGS workflows. This new format allows for use as typical labware; compatible with heat blocks, magnets, and thermocyclers. Both formats contain RFID for sample tracking, to be exclusively used with Covaris instruments.
Covaris Consumables Compatibility Guide
AFA-TUBE Features and Benefits
|Multiple processes in a single vessel (e.g. shearing, non-contact mixing, lysis)||Workflow simplification|
|Unique, engineered polymer for isothermal control||No heat-induced bias or sample damage|
|RFID-based sample plate tracking||Sample chain of custody and multi-plate, batch processing|
|Uniformly distributed cavitation with AFA-energetics®||Faster processing|
|Standard labware SBS-plate format design||Compatible with most liquid handlers|
|Laser etched plastics without fiber||Ease of automation|
AFA-TUBE NGS Workflows
Highly Accurate & Reproducible Results with AFA-TUBE
Extract HMW gDNA from Whole Blood
Narrow Size Distribution of gDNA Fragments from gDNA
Fig 4: Using the two different formats and sealing options, DNA was analyzed with the Fragment Analyzer after shearing on the LE220-plus. Data is shown for the 8 AFA-TUBE TPX Strip and 96 AFA-TUBE Plate for 175 bp and 350 bp for 10 μl. Results show high reproducibility and accuracy for all three configurations.
Highly Reproducible DNA Fragmentation Direct from Whole Blood
Fig 5: Graph shows highly reproducible DNA fragmentation profiles (30 μl of whole blood) with low Coefficient of Variation. DNA fragment size modes are between 1 and 1.3 kb. Shearing to NGS compatible fragment size distributions (150 to 500 bp) can be achieved by adjusting the AFA conditions (not shown). High molecular weight DNA (40 kb) can be extracted by adjusting extraction buffer conditions (e.g. truPOP buffer from Covaris). Processing time is 30 seconds per column for 96 AFA-TUBE TPX Plate.