cryoPREP Consumables

tissueTUBE

The tissueTUBE is a unique biomedical device constructed of strong, flexible plastic. When used in combination with an AFA® tube, tissueTUBE devices facilitate the closed vessel cryofracture of a biological tissue sample in a convenient, controlled, and easy-to-use manner. The film used in the tissueTUBE is made of Kapton, a “space-age” polymer originally developed to withstand the extreme temperature fluxes experienced in orbit. The tissueTUBE can be used with any of our cryoPREP Dry Pulverizers and allows you to extract target molecules in four easy steps: Load tissue in tissueTUBE, Snap (flash-freeze), Crack (dry pulverize), Pop (AFA-enabled cavitation to actively extract).

tissueTUBE TT1 (25)

tissueTUBEs for sample collection, storage, pulverization, and transfer. For samples of mass 1 g.

tissueTUBE TT2 (25)

tissueTUBEs for sample collection, storage, pulverization, and transfer. For samples of mass 2 g.

tissueTUBE TT05M (25)

TissueTubes for sample collection, storage, pulverization, and transfer. For samples of mass .05 g.

tissueTUBE TT1 Plug (25)

Plugs for use with the TT1 and TT1XT. Polypropylene with silicon O-ring for cryogenic storage.

tissueTUBE TT2 Plug (25)

Plugs for use with the TT2. Polypropylene with silicon O-ring for cryogenic storage.

tissueTUBE TT05M Plug (25)

tissueTUBE Plug for TT05M. Polypropylene with silicon O-ring for cryogenic storage.

tissueTUBE Workflow

  • Insert: Place the tissue in the center of the tissueTUBE.
  • Snap Freeze: Flash freeze the sample in liquid nitrogen to thermally stabilize biomolecules. Samples may be stored at -80° C.
  • Crack with cryoPREP: Dry pulverize the sample to increase surface area and disrupt extracellular matrix without the need for use of organic solvents. Samples may be stored at -80° C.
  • Pop with AFA: Acoustically extract biomolecule in an appropriate stabilizing buffer prior to purification or analysis.

tissueTUBE Features and Benefits

FEATUREBENEFIT
Non-contact processNo cross contamination or clean-up required
Streamlined tissue workflowsSingle-vessel collection, processing, transfer, and storage of tissue samples at cryogenic temperatures
Rapid cryogenic stabilization for all tissue typesNo tissue degradation of stable and labile molecules
Cryogenic pulverization into powder or fine particlesIncreased surface area for higher recoveries and multiple downstream applications