The tissueTUBE is a unique biomedical device constructed of strong, flexible plastic. When used in combination with an AFA® tube, tissueTUBE devices facilitate the closed vessel cryofracture of a biological tissue sample in a convenient, controlled, and easy-to-use manner. The film used in the tissueTUBE is made of Kapton, a “space-age” polymer originally developed to withstand the extreme temperature fluxes experienced in orbit. The tissueTUBE can be used with any of our cryoPREP Dry Pulverizers and allows you to extract target molecules in four easy steps: Load tissue in tissueTUBE, Snap (flash-freeze), Crack (dry pulverize), Pop (AFA-enabled cavitation to actively extract).
- Insert: Place the tissue in the center of the tissueTUBE.
- Snap Freeze: Flash freeze the sample in liquid nitrogen to thermally stabilize biomolecules. Samples may be stored at -80° C.
- Crack with cryoPREP: Dry pulverize the sample to increase surface area and disrupt extracellular matrix without the need for use of organic solvents. Samples may be stored at -80° C.
- Pop with AFA: Acoustically extract biomolecule in an appropriate stabilizing buffer prior to purification or analysis.
tissueTUBE Features and Benefits
|Non-contact process||No cross contamination or clean-up required|
|Streamlined tissue workflows||Single-vessel collection, processing, transfer, and storage of tissue samples at cryogenic temperatures|
|Rapid cryogenic stabilization for all tissue types||No tissue degradation of stable and labile molecules|
|Cryogenic pulverization into powder or fine particles||Increased surface area for higher recoveries and multiple downstream applications|