cryoPREP Consumables
tissueTUBE
The tissueTUBE is a unique consumable constructed of a strong, flexible polymer. When used in combination with an AFA-TUBE®, tissueTUBE Consumables facilitate the closed vessel cryofracture of biological tissue samples in a convenient, controlled, and easy-to-use manner.
The tissueTUBE is made of advanced polyimide film, a “space-age” polymer originally developed to withstand the extreme temperature fluxes experienced in orbit. Designed for use with cryoPREP Dry Pulverizers, the tissueTUBE enables extraction of target molecules in just four easy steps.
tissueTUBE TT1 (25)
tissueTUBEs for sample collection, storage, pulverization, and transfer. For samples of mass 1 g.
tissueTUBE TT2 (25)
tissueTUBEs for sample collection, storage, pulverization, and transfer. For samples of mass 2 g.
tissueTUBE TT05M (25)
TissueTubes for sample collection, storage, pulverization, and transfer. For samples of mass .05 g.
tissueTUBE TT1 Plug (25)
Plugs for use with the TT1 and TT1XT. Polypropylene with silicon O-ring for cryogenic storage.
tissueTUBE TT2 Plug (25)
Plugs for use with the TT2. Polypropylene with silicon O-ring for cryogenic storage.
tissueTUBE TT05M Plug (25)
tissueTUBE Plug for TT05M. Polypropylene with silicon O-ring for cryogenic storage.
tissueTUBE Workflow
- Insert: Place the tissue in the center of the tissueTUBE.
- Snap Freeze: Flash freeze the sample in liquid nitrogen to thermally stabilize biomolecules. Samples may be stored at -80° C.
- Crack with cryoPREP: Dry pulverize the sample to increase surface area and disrupt extracellular matrix without the need for use of organic solvents. Samples may be stored at -80° C.
- Pop with AFA: Acoustically extract biomolecule in an appropriate stabilizing buffer prior to purification or analysis.
tissueTUBE Features and Benefits
| FEATURE | BENEFIT |
| Non-contact process | No cross contamination or clean-up required |
| Streamlined tissue workflows | Single-vessel collection, processing, transfer, and storage of tissue samples at cryogenic temperatures |
| Rapid cryogenic stabilization for all tissue types | No tissue degradation of stable and labile molecules |
| Cryogenic pulverization into powder or fine particles | Increased surface area for higher recoveries and multiple downstream applications |