Focused-ultrasonicator Instruments and Cryoextraction Systems
Covaris Focused-ultrasonicators are engineered to meet your sample preparation needs by delivering highly-controlled and focused acoustic energy to your samples. Focused-ultrasonicators standardize sample prep for a variety of application areas including DNA/RNA shearing for Next-Gen sequencing, FFPE extraction, chromatin shearing, cell lysis, formulations, and more.
cryoPREP Dry Pulverizers are designed to integrate the tissue processing workflow from flash-freezing to extraction with AFA® Focused-ultrasonicators.
Covaris instruments can provide tunable DNA fragment sizes from 150 bp to 5 kbp. Random shearing with Adaptive Focused Acoustics (AFA) energy generates a log normal distributed DNA fragment profile. We provide protocols for the most commonly required DNA sizes. Click here to learn more about our DNA Shearing protocols. Typically, sizes between the defined protocols may be achieved by increasing the time of the treatment of the nearest size DNA fragment (upper side).
Purity of the water in the bath is very important for the proper acoustic coupling. When applying acoustic energy in rate-limited applications, foreign material such as algae and particulates may scatter the high frequency focused acoustic beam, resulting in a shift to a larger mean fragment size. Bath water should be deionized water, changed daily unless continuously treated by the optional Covaris Water Conditioning System (available for S, E, and LE instruments). Click to learn more about the WCS System.
No, the pulverized sample does not need to go through any sample processing immediately. After cryofracturing with the cryoPREP, tissueTUBE plugs can be used to seal the tissueTUBEs for long-term storage at -80° C, or vapor phase liquid nitrogen. Alternatively, the cryofractured tissue can be transferred into cryovials for long-term storage.