DNA Shearing Protocols for Ion Torrent Platforms with the M220 Focused-ultrasonicator
DownloadQuick Guide: DNA Shearing with M220 Focused-ultrasonicator
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M220 Focused-ultrasonicator
SKU
500295
- Treatment Power: 75 W PIP
- Sample Processing Capacity: Single Tube
- NGS Sample Volumes: 15 to 130 µl
The M220 Focused-ultrasonicator offers the “Scientist’s Standard” in a compact, easy-to-use system making it the ideal DNA shearing solution for MiSeq and PGM users. AFA® technology in the M220 eliminates operator-induced variations, improves performance, increases efficiency, and provides standardized results.
| Technical Specification | Description |
|---|---|
| Primary Applications | Cell lysis: mammalian, bacterial, yeast, cfDNA Extraction from Plasma, DNA Extraction from Dried Blood Spots, DNA/RNA/Chromatin Shearing, DNA/RNA/Total NA/Protein Extraction from FFPE, Tissue lysis and extraction |
| Treatment Power | 75 W PIP |
| Part Number | 500295 |
| Power Requirements | 100-240 VAC 500 VA, 50-60Hz |
| Operating Environment | Ambient temperature: 19 °C to 25 °C (66 °F to 77 °F) Relative humidity: 30% to 70% |
| Operating Temperature | Internal temperature control: Protocol temperature range from 7 °C to 20 °C |
| Safety | Complies with Low Voltage Directive 2006/95/EC. Certified to IEC/EN/UL 61010-1:2004 “Safety Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use, Part1: General Requirements" |
| Regulatory Labeling | CE, ETL Mark (for Product Safety), WEEE |
| SonoLab Version | SonoLab 7 |
| Operating System | Windows 10 |
| WCS | NA |
| Chiller | Solid state chiller built-in |
| Sample Processing Capacity | Single Tube |
| NGS Sample Volumes | 15 to 130 µl |
| Recommended Batch Size | NA |
| Covaris Consumables | microTUBE, microTUBE-50, milliTUBE 1 ml, miniTUBE |
| Upgrade Potential | No |
| Associated Accessories | M220 Accessories |
Key Features
- Controlled energy delivery
- Isothermal processing
- Non-contact, closed vessel
- Quiet operation
- Robust and reliable design
- Tried and tested in major genome centers worldwide
Benefits
- User-selectable fragment lengths
- No heat damage, high sample recovery
- No cross contamination, no aerosols, no clean up
- No discomfort to operators
- Reproducible results, year after year
- The mechanical shearing method of choice
DNA Shearing for Next Generation Sequencing (NGS) with the M220 Focused-ultrasonicator
DNA Shearing for NGS with the ME220 Focused-ultrasonicator.
DownloadMagnetic Bead-based Clean-up Using AFA-energetics®
When precisely tuned, this stream of Covaris Adaptive Focused Acoustic energy enables DNA shearing precisely and reliably to desired fragment sizes, in addition to gentle...
DownloadProtein Extraction from Yeast: Comparison of the Covaris Adaptive Focused Acoustics®(AFA) Process to Conventional Bead Beating and Probe Sonication
The efficiency of several mechanical-based lysis and extraction techniques, such as Adaptive Focused Acoustics (AFA), probe sonication, and bead beating from yeast isolates was compared.
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