M220 Focused-ultrasonicator

  • Treatment Power: 75 W PIP
  • Sample Processing Capacity: Single Tube
  • NGS Sample Volumes: 15 to 130 µl

The M220 Focused-ultrasonicator offers the “Scientist’s Standard” in a compact, easy-to-use system making it the ideal DNA shearing solution for MiSeq and PGM users. AFA® technology in the M220 eliminates operator-induced variations, improves recoveries, increases efficiency, and provides standardized results.

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Technical SpecificationDescription
Primary ApplicationsCell lysis: mammalian, bacterial, yeast, cfDNA Extraction from Plasma, DNA Extraction from Dried Blood Spots, DNA/RNA/Chromatin Shearing, DNA/RNA/Total NA/Protein Extraction from FFPE, Tissue lysis and extraction
Treatment Power75 W PIP
Part Number500295
Power Requirements100-240 VAC 500 VA, 50-60Hz
Operating EnvironmentAmbient temperature: 19 °C to 25 °C (66 °F to 77 °F) Relative humidity: 30% to 70%
Operating TemperatureInternal temperature control: Protocol temperature range from 7 °C to 20 °C
SafetyComplies with Low Voltage Directive 2006/95/EC. Certified to IEC/EN/UL 61010-1:2004 “Safety Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use, Part1: General Requirements"
Regulatory LabelingCE, ETL Mark (for Product Safety), WEEE
SonoLab VersionSonoLab 7
Operating SystemWindows 10
ChillerSolid state chiller built-in
Sample Processing CapacitySingle Tube
NGS Sample Volumes15 to 130 µl
Recommended Batch SizeNA
Covaris ConsumablesmicroTUBE, microTUBE-50, milliTUBE 1 ml, miniTUBE
Upgrade PotentialNo
Associated AccessoriesM220 Accessories

Key Features

  • Controlled energy delivery
  • Isothermal processing
  • Non-contact, closed vessel
  • Quiet operation
  • Robust and reliable design
  • Tried and tested in major genome centers worldwide


  • User-selectable fragment lengths
  • No heat damage, high sample recovery
  • No cross contamination, no aerosols, no clean up
  • No discomfort to operators
  • Reproducible results, year after year
  • The mechanical shearing method of choice

Quick Guide:

Quick Guide: DNA Shearing with M220 Focused-ultrasonicator


M220 Focused-ultrasonicator Setup & Instruction Manual

Product Inserts and User Manuals

Two New Solutions for Nucleic Acid Fragmentation


Five-Minute Ultrasonic Inactivation and Protein Extraction of Mycobacteria for Identification by MALDI-ToF MS


Protein Extraction of Non-Candida Yeast using an advanced acoustic technology for MALDI-TOF Identification


Evaluation of a New Protein Extraction Method Using an Advanced Acoustic Technology for Identification of Filamentous Fungi by MALDI-TOF MS


Four Minute Mycobacterium sp. Sample Preparation Process for MALDI-TOF Identification


Formulation and Drug Delivery Breakthrough processing for poorly soluble API’s


Covaris-certified Consumable Guide


M-Series Focused-ultrasonicators


AFA-based Efficient DNA Extraction and Optional Mechanical Shearing from Mycobacteria smegmatis

DNA Extraction from mycobacteria smegmatis.

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AFA-based MALDI Biotyper Sample Preparation for Mycobacteria Colonies


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Analysis of DNA fragments using the Agilent 2100 Bioanalyzer

DNA shearing protocols for Focused-ultrasonicators with the Agilent DNA 12000 kit was used to evaluate the average base pair size because of the large fragment...

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DNA Shearing for Next Generation Sequencing (NGS) with the M220 Focused-ultrasonicator

DNA Shearing for NGS with the ME220 Focused-ultrasonicator.

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Magnetic Bead-based Clean-up Using AFA-energetics®

When precisely tuned, this stream of Covaris Adaptive Focused Acoustic energy enables DNA shearing precisely and reliably to desired fragment sizes, in addition to gentle...

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Protein Extraction from Yeast: Comparison of the Covaris Adaptive Focused Acoustics®(AFA) Process to Conventional Bead Beating and Probe Sonication

The efficiency of several mechanical-based lysis and extraction techniques, such as Adaptive Focused Acoustics (AFA), probe sonication, and bead beating from yeast isolates was compared.

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Simplifying ChIP from Primary Cells and Clinical Samples for High-throughput Epigenetic Applications

Large-scale analysis of histone marks and transcription factor interactions uses high-quality chromatin, use of validated ChIP-grade antibodies, and optimized reagents to produce meaningful results.

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