Adaptive Focused Acoustics®(AFA®) for Pharmaceutical Formulation
AFA® powered drug formulation systems can improve a variety of pharmaceutical formulations. From early-stage discovery processing to continuous flow manufacturing with PAT feedback, AFA processing can dramatically shorten processing time, improve reproducibility, and construct formulations that are otherwise not possible.
AFA Technology for Pharmaceutical Formulation and Drug Delivery
Today, the majority (> 80%) of new chemical entities (NCE) discovered by the pharmaceutical industry are poorly soluble or lipophilic compounds; as are about 40% of existing drugs in the market. Consequently, this creates major challenges in drug development due to poor solubility, short biological half life, poor bioavailability, prominent adverse effects, and stability of NCE.
Adaptive Focused Acoustics® (AFA®) provides the ability to process a number of different constructs to help overcome solubility difficulties. Using AFA-based systems, both Liposomes and Emulsions are prepared at low processing temperatures without the use of organic solvents. This allows for the encapsulation of temperature or solvent sensitive APIs such as peptides or proteins, which would otherwise be destroyed or inactivated during the preparatory processing. Thus, API’s previously considered not feasible, can now be considered for development.
Download the Formulations Brochure
FEATURE | BENEFIT |
Isothermal | Eliminates molecular thermal damage by maintaining isothermal sample temperatures during the process |
Scalable | Scales from bench-top screens through clinical studies (microliters to 1,000+ liters) Compatible with Quality By Design and Process Analytical Technology |
Non-contact | Enables sterile, closed, single-use fluid circuits which eliminate carryover Enables disposable wetted surfaces which minimizes impurities |
Reproducible | Standardizes the process application of industry-proven AFA® technology Automated process by minimizing subjective operator influence |
Rapid | Improves processing performance (from 2 days to 2 minutes); increases solubility and stability |
AFA Enabled Applications for Pharmaceutical Formulations and Drug Delivery Systems
Micronization
Nanosuspension
Nanosuspensions have emerged as an effective strategy for the delivery of hydrophobic drugs because of the unique versatility and advantages in formulation. AFA can be used to create nanosuspensions through the complete life cycle from discovery through first human trials. By enabling closed vessel, contamination free wet milling in a scalable manner, early-stage compounds can be dosed and evaluated, without extensive Formulation development, with a very minimal amount of material. Subsequent trials can be completed with the same technology through the preparation of larger volumes of nanosuspension sample material.
The Covaris Focused Ultrasonicator has successfully demonstrated the ability to mill small volumes of early stage material into nanosuspensions suitable for animal dosing. Volumes as low as 100ul are possible with 100% recovery of the material.
SEM before and after wet milling
Crystallization
AFA can be used to control nucleation and crystal distribution. Other benefits of AFA technology for crystallization include:
- Control API “bottom up” nucleation and crystal growth in a batch vessel or continuous process stream
- Control particle size by influencing growth dynamics
- Accelerate kinetic energy reactions with extreme mixing velocities in isothermal conditions with a non-contact process
- Integrate PAT for automated, real time feedback, and Quality Control
Ibuprofen with AFA produces 700 nm particles while untreated samples are large, uneven clumps.
AFA-Liposome Formation
Liposomes are being used to deliver a number of different active pharmaceuticals for both small and large molecule API. Traditional liposome preparation methods include detergent depletion, ethanol injection, reverse-phase evaporation and emulsification. Processing methods include high-pressure homogenization, extrusion, and conventional ultrasound. The major disadvantages of these preparation methods include the consumption of large volumes of volatile organic solvents, multiple lengthy steps and degradation of the sample, and subsequent loss of bioactivity, due to heat exposure.
Adaptive Focused Acoustics® (AFA®) technology offers a breakthrough alternative to traditional methods, by forming monodisperse liposomes at 4°C (below the lipid phase transition temperature) and eliminating the need to use organic solvents.
Fully scalable from 100µL to continuous flow manufacturing, AFA-Liposome formation is possible across a range of lipids and formulations, including the addition of cholesterol and other difficult to load materials. Active ingredients can be loaded into the AFA-liposome at the time of formation, allowing a very simple and effective, one step formulation within a matter of minutes, without the risk of cross contamination or the need to cleanup.
FEATURE | BENEFIT |
Co-solvent free | No need to dissolve lipids or hydrophobic compounds in a co-solvent. All the materials can be included in the aqueous buffer, and then formulated. |
Low-Temperature Formulation | Liposomes can be formed at 4°C, irrespective of the lipid phase transition temperature. |
Delicate Compounds | Energy levels can be pre-set to enable formulation of delicate macromolecules such as siRNA and proteins. |
Short Processing Time | One-step formulation using an automated apparatus. |
No Cross Contamination | Liposomes are produced in single-use autoclavable vessels. |
Automated Size Control (under development) | Real-time monitoring and feedback control of particle size. |
Scalable | Capable of scaling from 100 µL to continuous flow manufacturing. |
AFA-NANOPARTICLE® Formation (Polymer based)
Nanoparticles have been shown to be effective Drug Delivery Systems because of their biocompatibility and biodegradability. Nanoparticles act as potential carries for several classes of drugs such as anticancer agents, antihypertensive agents, immunomodulators, and hormones; and macromolecules such as nucleic acids, proteins, peptides, and antibodies.
AFA can be used in the emulsification steps of PLGA nanoparticle formation, to achieve monodisperse particle size distributions as small as 80nm. Additionally, the intense yet highly controllable mechanical energy of AFA allows the use of mild solvents, such as triacetin, in AFA-Nanoparticle formation. Combined with inherent low temperature processing, AFA-Nanoparticles are formed using temperature and solvent sensitive materials that would degrade with traditional processing methods.
As presented at CRS 2013 annual meeting in Honolulu: “Contact-free Encapsulation of Proteins in PLGA Nanoparticles by Focused Ultrasound” Schiller, et al.
AFA-Nanoemulsion Formulation
Nanoemulsions have become an important delivery system for the controlled delivery of pharmaceuticals and cosmeceuticals, and for the optimized dispersion of active ingredients in skin layers, as well as ocular delivery of active molecules.
AFA allows the construction of tightly distributed nanoemulsions achieving average mean particle sizes as low as 20nm. Volumes ranging from 100µL through continuous flow processing are possible, and all processing is done in a closed, sterile vessel at a controlled temperature. Tight temperature control enables the encapsulation of sensitive biological molecules such as peptides and proteins, without degrading bioactivity due to heating that alternative methods require.
Particle size and distribution can be controlled automatically via the acoustic processing parameters, to maintain a high quality output product.
Technology | AFA | Microfluidizer | Self Assembling | Sonication |
Heat | Below room temp (<20°C) | 50-100°C | 60-100°C | Based on time of exposure and power of sonication |
Labor/Skill | Low | High | High | High |
Wastage | Low | High | Medium | Medium |
End product | Homogenous | Homogenous | Homogenous | NO |
Pressure | NO | High 24000-28000 psi | NO | Based on the time/power of procedure |
Closed Vial Process | YES | NO | NO | NO |
Cross contamination / Contamination | NO | YES | YES | YES |
Isothermal Processing | YES | NO | NO | NO |
Precise Energy Control | YES | NO | NO | NO |
Non Contact processing | YES | NO | NO | NO |
Heat damage to sample | NO | YES | YES | YES |
Repeatable without error | YES | NO | NO | NO |
Featured Products
E220 Focused-ultrasonicator
Versatile, high power system engineered for pre-analytical sample processing of 1 to 96 samples.
Pilot and Production-scale Focused-ultrasonicator System
Focused-ultrasonicator system for continuous flow processing of biological and chemical applications for formulations and drug delivery systems. Custom developed.
E220evolution Focused-ultrasonicator
High power, 1 to 8 sample batch processing instrument powered by AFA.
Resources
AFA-Liposome and AFA-Nanoemulsions Protocols
Protocols for AFA liposome preparation at 4°C and construction of tightly distributed nanoemulsions.
Crystallization Protocols
Protocols for using AFA to control nucleation and crystal distribution.
References
Micronization
- Nano-suspension Formulations- Adaptive Focused Acoustics for the Formulation of Suspensions & Nano-Suspensions
Authors: Srikanth Kakumanu, PhD, and Hames Bernhard. Published in Drug Development & Delivery | July/ August 2011 Vol 11 No6 - Technical Evaluation of Adaptive Focused Acoustics – Formulation solubility screening using AFA
Nanosuspension
- Poster Presentation GRC Preclinical Form and Formulation for Drug Discovery 2019: A DOE Approach for Nanosuspension Preparation for Poor Solubility Pharmaceutical Compounds Authors: Fenmei Zheng, Brian Michaels, and Yinqing Lin
- Poster Presentation AAPS 2012 Solidification of Nanosuspension via Spray Granulation.
Authors: Yidan Lan, Srikanth Kakumanu, Carl Beckett, and Nigel Langley
Demonstrated the feasibility of combining AFA technology (to produce nanosuspension by Tops down milling) and spray granulation. - Nano-suspension Formulations- Adaptive Focused Acoustics for the Formulation of Suspensions & Nano-Suspensions
Authors: Srikanth Kakumanu, PhD, and Hames Bernhard
Published in Drug Development & Delivery | July/ August 2011 Vol 11 No6
Crystallization
- Poster: Duarte et. al., AAPS 2017. Bottom-up particle engineering technologies: benchmarking of co-precipitation processes for solubility enhancement and taste masking
- Poster: Kakumanu. AAPS 2017. Covaris Adaptive Focused Acoustics TM (AFA), a Novel Technology to Control Nucleation and Crystal Growth in a Mixed Suspension Mixed Product Removal (MSMPR) Crystallizer
- Presentation: 2017, Carl Beckett, VP Business Development, Covaris. Utilizing Adaptive Focused Acoustics (AFA) Technology to Control Nucleation and Crystal Distribution During API Crystallization
- Poster Abstract: Beckett et.al., AIChE Annual Meeting 2015. Continuous nanoparticle production using focused acoustics
- Poster: He et al., Flow Chemistry Congress 2013. Controlling Crystallization with Adaptive Focused Acoustics
- Master’s Degree Thesis: Alexandra Franzén, Department of Chemistry, Lund University (2011). Crystal Nucleation of Poorly Soluble Drugs: Experimental Results and New Knowledge
Liposomes
- Focused Ultrasound - A Novel Tool for Liposome Formulation (2012)
Authors: Srikanth Kakumanu, PhD, and Avi Schroeder, PhD
Published in Drug Development & Delivery - Making unilamellar liposomes using focused ultrasound. Tejera-Garcia et al. 2011. Langmuir 27:10088-97.
- Oxidized phosphatidylcholines facilitate phospholipid flip-flop in liposomes. Volinsky et al. 2011. Biophysical Journal 101:1376-84.
- Peptide-mediated targeting of liposomes to TrkB receptor-expressing cells. Ranjan et al. 2012. International Journal of Nanomedicine 7: 3475–85.
- Internalization of liposome nanoparticles functionalized with TrkB ligand in rat cochlear cell populations. Zou et al. 2008. European Journal of Nanomedicine 2:7–13.
- Freeze Fracture Electron Microscopic Examination of Liposomes Produced by Covaris AFA Technology. Kakumanu and Jain 2013. Drug Delivery Technology 13:46-50.
- Sensitivity of nanostructure in charged cubosomes to phase changes triggered by ionic species in solution. Liu et al. 2013. Langmuir 29:14265-73.
Nanoparticle Formulation
- Contact-free Encapsulation of Proteins in PLGA Nanoparticles by Focused Ultrasound. Schiller, Hanefeld, Schneider, Weigandt, Lehr. 40th Annual Meeting and Exposition of the Controlled Release Society, July 21-24, 2013, Honolulu, Hawaii
Nanoemulsion Formation
- Contact-free Encapsulation of Proteins in PLGA Nanoparticles by Focused Ultrasound. Schiller, Hanefeld, Schneider, Weigandt, Lehr. 40th Annual Meeting and Exposition of the Controlled Release Society, July 21-24, 2013, Honolulu, Hawaii
- A simple, scalable, isothermal process for nanoemulsion formation. Kakumanu, Schmitt, He, Beckett, Laugharn. 40th Annual Meeting and Exposition of the Controlled Release Society, July 21-24, 2013, Honolulu, Hawaii