Consumables - Tubes and Vials
Covaris tubes come in a variety of types and sizes for sample analysis and processing, largely used with AFA-energetics®. The Covaris Adaptive Focused Acoustics® (AFA®) process employs focused bursts of tunable acoustic energy through a water bath to a small focal zone within the Covaris tube immersed in the bath.
The Covaris tube is a critical component to the success of AFA. The AFA-TUBE®, microTUBE, milliTUBE and miniTUBE product lines were engineered by Covaris to provide controlled isothermal and repeatable treatment at all power settings.
|Fragment Size*||Sample Volume||Covaris Products|
|175 to 1.5 kbp||5 to 50 µl||AFA-TUBE|
|150 to 1.5 kbp||130 µl||microTUBE-130|
|150 to 1.5 kbp||55 µl||microTUBE-50|
|150 to 1.5 kbp||15 µl||microTUBE-15|
|2 kbp||200 µl||miniTUBE Clear|
|3 kbp||200 µl||miniTUBE Blue|
|5 kbp||200 µl||miniTUBE Red|
|6 – 20 kb||150 µl||g-TUBE|
*For validated fragment sizes, refer to the Covaris Focused-ultrasonicator Protocol.
A specially designed, laser etched polymer vessel enabling a single-tube workflow with no transfer steps for high-throughput workflows.
Optimized for use with Focused-ultrasonicators to reproducibly shear genomic DNA into fragments ranging from 2 to 5 kb in size.
A single-use tube that shears DNA using a bench-top centrifuge- no AFA involved. DNA shearing fragments 6-20kb.
The fiber inside the tubes serves a dual purpose. The first is providing nucleation sites for inertial cavitation. Cavitation is a process where a bubble in the liquid rapidly collapses creating a microjet that fragments the DNA. The second purpose is to allow for efficient mixing of the sample during processing. The acoustic fiber is thoroughly cleaned during the manufacturing process before being inserted inside the tubes and is free from organic contaminants.
Covaris has developed DNA shearing protocols using the microTUBE-500 that generates an equivalent DNA fragment size distribution as the one generated in T6 tubes when processed with AFA-energetics®. These protocols are available for all Covaris focused-ultrasonicators. Click here to find all of our DNA Shearing protocols for the latest information.