DNA Shearing Quick Guide: microTUBE-15 with S220/E220 Focused-ultrasonicator.
DownloadDNA Shearing with microTUBEs (<1.5kb fragments) S220/E220
DownloadQuick Guide: DNA Shearing with E220 Focused-ultrasonicator
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E220 Focused-ultrasonicator
SKU
500239
- Treatment Power: 100 W Avg. Incident Power
- Sample Processing Capacity: Single Tube
- NGS Sample Volumes: 15 to 130 µl
The E220 Focused-ultrasonicator enables multi-sample batch preparation, capable of processing a wide range of sample types and volumes. The E220 may be programed to process from 1 to 96 samples in a single batch.
| Technical Specification | Description |
|---|---|
| Primary Applications | Cell lysis: mammalian, bacterial, yeast, cfDNA Extraction from Plasma, DNA Extraction from Dried Blood Spots, DNA/RNA/Chromatin Shearing, DNA/RNA/Total NA/Protein Extraction from FFPE, Tissue lysis and extraction |
| Treatment Power | 100 W Avg. Incident Power |
| Part Number | 500239 |
| Power Requirements | 100-120, 200-240 V~ 50-60Hz, 500 VAC |
| Operating Environment | Ambient temperature: 19 °C to 25 °C (66 °F to 77 °F) Relative humidity: 30% to 70% |
| Operating Temperature | 0.5 °C to 55.0 °C |
| Safety | Complies with Low Voltage Directive 2006/95/EC. Certified to IEC/EN/UL 61010-1:2004 “Safety Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use, Part1: General Requirements |
| Regulatory Labeling | CE and ETL Mark |
| SonoLab Version | SonoLab 7 |
| Operating System | Windows 10 |
| WCS | Yes |
| Chiller | Chiller/heater re-circulating system-Not included,sold separately |
| Sample Processing Capacity | Single Tube |
| NGS Sample Volumes | 15 to 130 µl |
| Recommended Batch Size | 1 to 96 Samples |
| Covaris Consumables | microTUBE 8-strip (rack required), microTUBE 96 plate |
| Upgrade Potential | No |
| Associated Accessories | E220 Accessories |
Key Features
- Isothermal processing
- Non-contact, closed vessel
- Precisely controlled and adjustable energy delivery
- Automation ready
- Multi-sample capability
- Broad sample volume range
Benefits
- No heat damage, higher recovery
- No cross contamination, no aerosols, no clean up
- Standardized processes, highly reproducible
- Integrates into customers’ sample processing workflow
- Higher productivity, less operator intervention
- One instrument for multiple applications
Optimizing a Dual Fixation Protocol to Study Protein Complexes Binding to Chromatin in vivo
This application note provides shearing profiles, evaluates epitope integrity each shearing time and ChIP-qPCR results obtained using single step formaldehyde fixation and dual crosslinking protocol.
DownloadStreamlined Ultra Low Sample Input and Processing Volume Chromatin Shearing Protocols for Fly Embryos and Mammalian Cell Lines
These protocols have been optimized for both mammalian cell lines and fly embryos and reliably fragment chromatin in low volumes from down to 10,000 mammalian...
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