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Watch On-Demand: Robust Extraction of Metabolites, Lipids, and Proteins from Tissues Using the Covaris R230
Professor, Department of Hematology and Hematopoietic Cell Transplantation, City of Hope
City of Hope’s Lorenzi Lab is building toward a systems-level biochemical picture of host and microbiome biology by extracting DNA, RNA, protein, metabolites, and lipids from the same biological sample. In this presentation, Dr. Phil Lorenzi details a sequential multiomic extraction tissue workflow built on the Covaris R230 ultrasonicator, tested across three challenging matrices: mouse liver, mouse skeletal muscle, and human stool. Using matched extraction solvents for each analyte class — including trifluoroethanol for metabolites, ethanol-based solvents for lipids, and urea-based buffers for proteins — the team evaluated recovery, reproducibility, and class-specific lipid annotation across nine workflow variations.
Metabolite recovery proved largely consistent regardless of workflow, while lipid recovery varied significantly based on extraction solvent polarity, with nonpolar, ethanol-based solvents yielding the best results. Protein recovery, however, declined notably when metabolite and lipid extraction preceded it, signaling a clear area for further optimization. With the R230’s capacity to process 96 samples simultaneously, the lab is now scaling this sequential multiomic extraction tissue approach to a 69-mouse aging study spanning 15 tissues per animal, generating early correlative insights between metabolite signatures, cytokine profiles, and gut microbiome diversity.