truCOVER DNA Library Prep Kits User Manual
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truCOVER® DNA Library Prep Kits
SKU
520361KIT/520366KIT
- Sample Type: DNA from blood, saliva, FFPE, cell culture, and microbes, among other sources
- Application: Whole Genome Sequencing with or without Amplification, Compatible with Whole Exome Sequencing and Targeted Gene Panel workflows
Covaris truCOVER® DNA Library Prep Kits deliver unmatched flexibility and performance. Prepare high-quality libraries from as little as 0.1 ng or as much as 500 ng of DNA across a wide range of input amounts. Whether working with blood, saliva, buccal swabs, FFPE, cell culture, or microbial DNA, truCOVER ensures reliable and reproducible creation of NGS libraries with a single, streamlined workflow.
Precision WGS library prep to streamline your NGS workflow
Tunable mechanical fragmentation powered by Adaptive Focused Acoustics® (AFA®) technology delivers precise control over insert size across diverse sample types and GC content, supporting uniform coverage, reproducible library complexity, and more efficient use of sequencing reads. Optimized reagents further streamline the workflow to reduce variability and protect overall sequencing investment.
Looking for RNA applications? Explore truCOVER® Total RNA Library Prep Kits designed for degraded and low-input RNA samples.
Key Features of truCOVER DNA Library Prep Kits:
- High-quality library yield, with negligible duplication rate and insert size bias. Yields can be boosted up to 6x through 4x amplification without compromising fragment integrity.
- Consistent and uniform library sizes via easily tunable fragmentation, even for low-input or degraded samples
- Operational efficiency (e.g., 30% reduced turnaround time) via simplified workflow. High-quality library generation in just 2 hours and 50 minutes for input samples ≥50 ng with 25 minutes of hands-on time. Libraries from low-input samples needing amplification can be constructed in 3 hours and 50 minutes with 35 minutes of hands-on time.
- Sequence-independent uniform and unbiased libraries and batch-to-batch consistency enables optimized flow cell utilization
- Wide sample input range (0.1 - 500 ng) across sample types (blood, saliva, FFPE, cell culture and microbial DNA) in one workflow with or without amplification, adding workflow flexibility
- Specially formulated clean-up reagents included in the kit, which is designed to reduce downstream sequencing instrument fragment size bias, enabling simplified library pooling by mass or equal volume without additional steps
truCOVER Workflow with Optional Amplification
![]({{media url=".renditions/wysiwyg/Box_A_and_B_PCR-Free_Kit.png"}})
truCOVER WGS PCR-Free Library Prep Kit Contents
The truCOVER WGS PCR-free Library Prep Kit (520361KIT) contains enough reagents to process 96 samples of 50 – 500 ng without the need for amplification.
|
Box A (Store at -25 to -15° C) |
Box B (Store at 2 - 8° C) |
| Enzyme Mix and Enzyme Buffer | Bead Purification Reagent |
| Ligase and Ligase Buffer | DNA Elution Buffer |
truCOVER Library Amplification Kit Contents
The truCOVER Library Amplification Kit (520366KIT) is an add-on kit for processing low-input samples from 0.1 – 50 ng. Order the truCOVER DNA Library Prep Kit with Amplification (520361KIT and 520366KIT) for the complete library preparation of low-input samples.
|
Box A (Store at -25 to -15° C) |
Box B (Store at 2 to 8° C) |
| Amplification Master Mix | Bead Purification Reagent |
| P5/P7 Primer Mix | DNA Elution Buffer |
Want to learn more? Contact us to speak with a representative.
| Technical Specification | Description |
|---|---|
| Part Number | 520361KIT truCOVER PCR-free Library Prep Kit 520366KIT truCOVER Amplification Kit |
| Sample Input | 50 – 500 ng PCR-free Applications 0.1 – 50 ng Applications with Amplification |
| Sample Type | DNA from blood, saliva, FFPE, cell culture, and microbes, among other sources |
| Application | Whole Genome Sequencing with or without Amplification, Compatible with Whole Exome Sequencing and Targeted Gene Panel workflows |
| Instrument Compatibility | All Focused-ultrasonicators |
| Sample Processing Per Kit | 96 |
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