truChIP® Chromatin Shearing Kit Protocol
DownloadtruChIP® Chromatin Shearing Kit
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truChIP Chromatin Shearing Kit with Formaldehyde
SKU
520154
- Application: Chromatin Shearing
The truChIP Chromatin Shearing Kit with Formaldehyde is designed and optimized for the efficient and reproducible shearing of chromatin from cultured mammalian cells (suspension or adherent) using Covaris Adaptive Focused Acoustics (AFA) technology.
Kit contains truChlP reagents, formaldehyde, and protocol for chromatin shearing of 10-30mm cells (High Cell; 15 samples) or 1-3mm cells (Low Cell; 50 samples). To be used with milliTUBE with AFA Fiber or microTUBE with AFA Fiber.
| Technical Specification | Description |
|---|---|
| Size | 1 |
| Part Number | 520154 |
| Application | Chromatin Shearing |
| Case | No |
Optimizing a Dual Fixation Protocol to Study Protein Complexes Binding to Chromatin in vivo
This application note provides shearing profiles, evaluates epitope integrity each shearing time and ChIP-qPCR results obtained using single step formaldehyde fixation and dual crosslinking protocol.
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