The t-PREP System is specifically engineered for rapidly processing small tissue samples in the 1-10 mg mass range. The t-PREP System integrates the entire sample collection, cryopreservation, storage, cryofracture, and homogenization for the controlled the extraction of biomolecules in a single vessel providing several benefits; increased yield, reduced workflow time, isothermal processing, and no sample loss.
Manual Cryoprep Impactor specifically designed for use with the t-PREP devices. Delivers a 1.3 J force to t-PREP to cryofracture tissue samples
High yield and reproducible protein extraction from small tissue samples
Total protein was extracted into 270 μl of Total Protein Extraction Buffer B from ~15 mg of mouse heart muscle using either the t-PREP Biomarker Extraction System or a dounce homogenizer. Mean protein yield was 182.7 μg protein/mg tissue for the t-PREP system compared to 68.2 μg protein/mg tissue.