Consumables - Tubes and Vials
miniTUBE
The miniTUBE is optimized for use with Focused-ultrasonicators to reproducibly shear genomic DNA into fragments ranging from 2 to 5 kb in size. Each type of miniTUBE (clear, blue or red) has a specific textured surface to provide the required shearing forces when used with the recommended AFA® settings. The shearing force is gentle and controlled to generate large DNA fragment with a tight fragment size distribution.
LOW-THROUGHPUT
MID-THROUGHPUT
HIGH-THROUGHPUT
miniTUBE Compatibility Guide
miniTUBE Compatibility Guide
miniTUBE Compatibility Guide
miniTUBE Features and Benefits
| FEATURE | BENEFIT |
| Continuous isothermal control | No heat damage so sample quality is preserved |
| Non-contact, closed vessels | No cross contamination, clean-up, or aerosol formation |
| Accurate, Precise, Reproducible, and Robust | Processes and protocols are highly reproducible delivering exactly the size distribution needed for your application, so you have more DNA exactly the size you need with every experiment |
| Gentle | DNA is undamaged providing higher yields and better sensitivity in all downstream applications |
| Non-biased | Mechanical shearing delivers DNA fragments unbiased by sequence context, GC content, or other factors, providing the best template for all sequencing applications |
miniTUBE performance & applications
The performance of the miniTUBEs is highly reproducible and concentration independent. 2 replicates of 4 different concentrations of Lambda DNA were processed using the same settings. Results are from 0.6% agarose gel (insert) and Agilent Bioanalyzer 12k chip.
Fig 1: miniTUBE Clear 2 kb
Fig 2: miniTUBE Blue 3 kb
Fig 3: miniTUBE Red 5 kb
