Company

Partners

Covaris works closely with industry leaders in life sciences to bring high-quality sample prep solutions to key application areas. Covaris AFA® (Adaptive Focused Acoustics) technology is the basis for reproducible, isothermal, non-contact sample preparation.

Partner Protocols

Covaris partners use AFA to ensure quality and consistency because sample preparation is the critical first step in achieving high-quality analytical results. We are proud to be included in their protocols as an important step in their workflows.

To learn more about partnership opportunities at Covaris, please contact Rich Doverspike, Executive Vice President, at [email protected]

NGS Sample Preparation

Recommended instruments: M220 or LE220-plus
Recommended consumable: microTUBE AFA Fiber with Crimp-Cap (PN [520052]) or Pre-Slit Snap-Cap (PN [520045]) (for use with an M220 Focused-ultrasonicator)

Illumina TruSeq DNA Nano

Recommended instruments: M220 or LE220-plus
Recommended consumable: microTUBE AFA Fiber with Crimp-Cap (PN [520052]) or Pre-Slit Snap-Cap (PN [520045]) (for use with an M220 Focused-ultrasonicator)

Illumina TruSeq DNA Exome

Recommended instruments: M220 or LE220-plus
Listed consumables: microTUBE AFA Fiber with Crimp-Cap (PN [520052] & PN [520052]), Pre-Slit Snap-Cap (PN [520045]), 8 microTUBE strip (PN [520053]), 96 microTUBE plate (PN [520078]), microTUBE AFA Fiber Screw-Cap (PN [520096])

FFPE Sample Processing

Illumina:

Illumina TruSight Tumor 170

View Protocol

Check out our application note, Covaris DNA Shearing Guide for Illumina TruSight Tumor 170 NGS Assay

Covaris Products Listed:

Covaris Focused-ultrasonicator, E220evolution (PN 500429) or LE220* (PN 500219)
Covaris 8 microTUBE Strip, PN 520053
Rack E220evolution 8 microTUBE Strip Adaptor, PN 500430
Rack 12 place 8 microTUBE Strip adaptor (for use with LE220), PN 500191

*Please Note: Covaris recommends the LE220-plus (PN 500569) in place of the LE220

ProtiFi

Covaris and ProtiFI^TM have partnered to address challenges encountered when performing protein extraction from archived FFPE tissues. The partnership led to the development of a simple, efficient, and optimized proteomics workflow enabled by Covaris and ProtiFI. The Covaris Adaptive Focused Acoustics (AFA) technology combined with the Covaris truXTRAC® FFPE Protein Extraction Kit drastically improves the sample prep by actively removing more paraffin while rehydrating tissue to isolate proteins prior to purification. ProtiFi, a protein purification technology, has developed S-Trap^TM columns to trap AFA-released proteins and digest them prior to analysis.

View more information on the ProtiFi S-TRAP

NanoString Technologies:

NanoString Technologies nCounter® XT Assay User Manual

View Protocol

For the most accurate results when using FFPE samples, NanoString recommends fragmentation via Covaris AFA technology, with an optimal target size between 200–300 bases.

Covaris Products Listed:

Covaris AFA Instrument (various models). Covaris recommends the M220 (PN 500295) or the LE220-plus (PN 500569) Contact us to learn what instrument is best for your research.

Methyl-Seq

Illumina TruSeq Methyl Capture EPIC Library Prep Kit

View Protocol

Covaris Products Listed:

microTUBE AFA Fiber Pre-Slit Snap-Cap, PN 520045
microTUBE Strips (8), package of 12, PN 520053
Covaris Focused-ultrasonicator M220, PN 500295

Agilent SureSelectXT Methyl-Seq Target Enrichment System for Illumina Multiplexed Sequencing

View Protocol

Covaris Products Listed:

Covaris Sample Preparation System, S-series* or E-series* model
96 microTUBE plate, PN 520078
microTUBE for individual sample processing, PN 520045

*Please note: Newer instruments have been released since this protocol was published. Covaris recommends the M220 (PN 500295) in place of an S-Series instrument, and the LE220-plus (PN 500569) in place of an E-Series instrument

Swift Biosciences ACCEL-NGS® METHYL-SEQ DNA LIBRARY KIT

View Protocol

This kit has been specifically validated using Covaris®-fragmented DNA. Contact us to learn what the best Covaris DNA fragmentation method is for you.

NuGEN Ovation Ultralow Methyl-Seq Library Systems

View Protocol

Covaris Products Listed:

Covaris Focused-ultrasonicator, S-series*

*Please note: Newer instruments have been released since this protocol was published. Covaris recommends the M220 (PN 500295) in place of an S-Series instrument

Snap-cap microTUBE, PN 520045

RNA-Seq

NuGEN Ovation^® RNA-Seq System V2

View Protocol

Covaris Products Listed:

Covaris Focused-ultrasonicator, S-series*

*Please note: Newer instruments have been released since this protocol was published. Covaris recommends the M220 (PN 500295) in place of an S-Series instrument