milliTUBE 1 ml AFA Fiber(100)

Cross-linked Yeast Chromatin Shearing on the Covaris Focused-ultrasonicator

The Broad Institute developed a cross-linked chromatin shearing protocol for yeast samples that utilizes the Covaris E210 Focused-ultrasonicator, to be used prior to immunoprecipitation.

Optimizing a Dual Fixation Protocol to Study Protein Complexes Binding to Chromatin in vivo

This application note provides shearing profiles, evaluates epitope integrity each shearing time and ChIP-qPCR results obtained using single step formaldehyde fixation and dual crosslinking protocol.

Simplifying ChIP from Primary Cells and Clinical Samples for High-throughput Epigenetic Applications

Large-scale analysis of histone marks and transcription factor interactions uses high-quality chromatin, use of validated ChIP-grade antibodies, and optimized reagents to produce meaningful results.