milliTUBE 1 ml AFA Fiber(100)

SKU
520130
  • Sample Volume: 1 ml
  • Application: Chromatin Shearing

12x12 mm tube with AFA Fiber; PTFE seal cap (volume 1ml). Rated for 1MHz, 150W PIP.

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Technical SpecificationDescription
Size100
ApplicationChromatin Shearing
Part Number520130
Mechanical Shearing PrincipleAFA®
Sample Volume1 ml
Polymer or GlassGlass
Instrument CompatibilityE220, E220evo, M220, S220

M220 Holder XTU
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M220 Holder XTU Insert milliTUBE 1 ml
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Rack E220e 4 Place milliTUBE 1ml
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ME220 Rack - 4 place milliTUBE 1 ml
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Optimizing a Dual Fixation Protocol to Study Protein Complexes Binding to Chromatin in vivo

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Cross-linked Yeast Chromatin Shearing on the Covaris Focused-ultrasonicator

The Broad Institute developed a cross-linked chromatin shearing protocol for yeast samples that utilizes the Covaris E210 Focused-ultrasonicator, to be used prior to immunoprecipitation.

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Optimizing a Dual Fixation Protocol to Study Protein Complexes Binding to Chromatin in vivo

This application note provides shearing profiles, evaluates epitope integrity each shearing time and ChIP-qPCR results obtained using single step formaldehyde fixation and dual crosslinking protocol.

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Simplifying ChIP from Primary Cells and Clinical Samples for High-throughput Epigenetic Applications

Large-scale analysis of histone marks and transcription factor interactions uses high-quality chromatin, use of validated ChIP-grade antibodies, and optimized reagents to produce meaningful results.

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