Optimizing a Dual Fixation Protocol to Study Protein Complexes Binding to Chromatin in vivo
12x12 mm tube with AFA Fiber; PTFE seal cap (volume 1ml). Rated for 1MHz, 150W PIP.
Technical Specification | Description |
---|---|
Size | 100 |
Application | Chromatin Shearing |
Part Number | 520130 |
Mechanical Shearing Principle | AFA® |
Sample Volume | 1 mL |
Polymer or Glass | Glass |
Instrument Compatibility | E220, E220evo, M220, S220 |
Cross-linked Yeast Chromatin Shearing on the Covaris Focused-ultrasonicator
The Broad Institute developed a cross-linked chromatin shearing protocol for yeast samples that utilizes the Covaris E210 Focused-ultrasonicator, to be used prior to immunoprecipitation.
Learn MoreOptimizing a Dual Fixation Protocol to Study Protein Complexes Binding to Chromatin in vivo
This application note provides shearing profiles, evaluates epitope integrity each shearing time and ChIP-qPCR results obtained using single step formaldehyde fixation and dual crosslinking protocol.
Learn MoreSimplifying ChIP from Primary Cells and Clinical Samples for High-throughput Epigenetic Applications
Large-scale analysis of histone marks and transcription factor interactions uses high-quality chromatin, use of validated ChIP-grade antibodies, and optimized reagents to produce meaningful results.
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