microTUBE

The Covaris microTUBE is used extensively for DNA shearing for generating deep coverage, unbiased fragments for advanced sequence analysis. The microTUBE was designed to optimize inertial cavitation and focus acoustic energy in small volumes (e.g., 50 ml). The integrated AFA® fiber provides nucleation sites and allows for both uniform and faster sample processing. The microTUBE is made with a special grade of hardened glass for low acoustic impedance.

8 microTUBE-50 AFA Fiber H Slit Strip V2

A specially designed and engineered glass vessel with H Slit septa to optimize DNA Shearing in a 50 μL sample volume with Covaris Focused-ultrasonicators.

8 microTUBE-130 AFA Fiber H Slit Strip V2

A specially designed and engineered glass vessel with H Slit septa to optimize DNA Shearing in a 130 μL sample volume with Covaris Focused-ultrasonicators.

microTUBE-130 AFA Fiber Screw-Cap

For single-tube processing, optimized for sample volume of 130 μl

microTUBE-500 AFA Fiber Screw-Cap (25)

Covaris certified glass consumables for sample volume of 500 μl. Ideal for FFPE extraction.

8 microTUBE-15 AFA Beads H Slit Strip V2

microTUBE-15 incorporates AFA Beads which enable fully controllable and repeatable DNA shearing, sample volume 15 μl.

96 microTUBE-50 AFA Fiber Plate

96 microTUBE-50 with AFA Fiber in a single use pre-pierced plate, pre-assembled, ready to use. Optimized for 50 µl sample volume, automation friendly.

microTUBE Compatibility Guide

M220

S220

ME220

E220evo

E220

LE220/LE220-plus/LE220R-plus

microTUBE Features and Benefits

FEATUREBENEFIT
IsothermalNo heat damage, so sample quality and labile metabolites are preserved
Non-contact, closed vesselNo cross contamination, clean-up, or aerosol formation
Accurate, Precise, and reproducibleProcesses and protocols are highly reproducible delivering exactly the size distribution needed for your application, so you have more DNA exactly the size you need with every experiment
GentleDNA is undamaged providing higher yields and better sensitivity in all downstream applications
UnbiasedMechanical shearing delivers DNA fragments unbiased by sequence context, GC content, or other factors, providing the best template for all sequencing applications
Higher YieldDelivering sheared DNA of the optimal size for your sequencer, by unbiased, and non-damaging (eg, heat damage), means there is DNA and RNA ready for your library preparation when you shear by AFA in a microTUBE

microTUBE performance & applications

Highly Versatile



Fig 1: DNA samples treated with different AFA treatments and analyzed with Agilent Bioanalyser 12k chip

DNA Concentration Independent



Fig 2: DNA samples of different concentration treated with the same AFA treatments and analyzed with Agilent Bioanalyser 12k chip

Highly reproducible across a 96 well microTUBEs plate



Fig 3: 96 samples processed following a 200bp treatment in a Covaris 96 microTUBEs plate and analyzed with Caliper GX 1k



Fig 4: 96 samples processed following a 200bp treatment in a Covaris 96 microTUBEs plate and analyzed with Caliper GX 1k. Peak sizes are plotted for each sample as a function of their position in a row and column.

Reproducible RNA shearing



Fig 5: Total RNA Fragmentation. Agilent Bioanalyzer electropherogram of 5ug of mRNA processed in a volume of 130ul of TE in a microTUBE according to the operating conditions. Mean fragment size is observed at 200 bases.



Fig 6: mRNA Fragmentation. Agilent Bioanalyzer electropherogram of 100ng of mRNA processed in a volume of 130ul of TE in a microTUBE according to the operating conditions. Mean fragment size is observed at 400 bases.