truChIP Chromatin Shearing Kit with Formaldehyde
SKU
520154
- Application: Chromatin Shearing
The truChIP® Chromatin Shearing Kit with Formaldehyde is designed and optimized for the efficient and reproducible shearing of chromatin from cultured mammalian cells (suspension or adherent) using the Covaris Adaptive Focused Acoustics® (AFA®) technology.
Kit contains truCHlP reagents, formaldehyde, and protocol for chromatin shearing of 10-30mm cells (High Cell; 15 samples) or 1-3mm cells (Low Cell; 50 samples). To be used with milliTUBE with AFA Fiber or microTUBE with AFA Fiber.
| Technical Specification | Description |
|---|---|
| Part Number | 520154 |
| Application | Chromatin Shearing |
| Instrument Compatibility | LE220-plus, LE220R-plus, ME220, E220, E220evo, M220, S220 |
| Case | No |
Optimizing a Dual Fixation Protocol to Study Protein Complexes Binding to Chromatin in vivo
Posters
DownloadOptimizing a Dual Fixation Protocol to Study Protein Complexes Binding to Chromatin in vivo
This application note provides shearing profiles, evaluates epitope integrity each shearing time and ChIP-qPCR results obtained using single step formaldehyde fixation and dual crosslinking protocol.
Learn MoreSimplifying ChIP from Primary Cells and Clinical Samples for High-throughput Epigenetic Applications
Large-scale analysis of histone marks and transcription factor interactions uses high-quality chromatin, use of validated ChIP-grade antibodies, and optimized reagents to produce meaningful results.
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