truChIP Chromatin Shearing Kit with Formaldehyde

SKU
520154
  • Application: Chromatin Shearing

The truChIP® Chromatin Shearing Kit with Formaldehyde is designed and optimized for the efficient and reproducible shearing of chromatin from cultured mammalian cells (suspension or adherent) using the Covaris Adaptive Focused Acoustics® (AFA®) technology.

Kit contains truCHlP reagents, formaldehyde, and protocol for chromatin shearing of 10-30mm cells (High Cell; 15 samples) or 1-3mm cells (Low Cell; 50 samples). To be used with milliTUBE with AFA Fiber or microTUBE with AFA Fiber.

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Technical SpecificationDescription
Part Number 520154
ApplicationChromatin Shearing
Instrument CompatibilityLE220-plus, LE220R-plus, ME220, E220, E220evo, M220, S220
CaseNo

Quick Guide:

truChIP® Chromatin Shearing Kit

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Safety Data Sheet Reverse Crosslinking Buffer

Safety Data Sheets
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Safety Data Sheet Protease Inhibitor Cocktail

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Chromatin Brochure

Application Notes, Brochures
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Optimizing a Dual Fixation Protocol to Study Protein Complexes Binding to Chromatin in vivo

Posters
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Safety Data Sheet 16% Formaldehyde, Methanol Free

Safety Data Sheets
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Safety Data Sheet Halt Protease Inhibitor Cocktail

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Safety Data Sheet 10X Fixing Buffer

Safety Data Sheets
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Safety Data Sheet 10X SDS Shearing Buffer D3

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Safety Data Sheet 5X Lysis Buffer

Safety Data Sheets
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Safety Data Sheet 2X IP Dilution Buffer 3X IP Dilution Buffer

Safety Data Sheets
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Safety Data Sheet 1X Quenching Buffer

Safety Data Sheets
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Comprehensive Epigenomic Sample Prep Workflow Solutions

Brochures
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truChIP® Chromatin Sample Preparation Solutions

Brochures
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Optimizing a Dual Fixation Protocol to Study Protein Complexes Binding to Chromatin in vivo

This application note provides shearing profiles, evaluates epitope integrity each shearing time and ChIP-qPCR results obtained using single step formaldehyde fixation and dual crosslinking protocol.

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Simplifying ChIP from Primary Cells and Clinical Samples for High-throughput Epigenetic Applications

Large-scale analysis of histone marks and transcription factor interactions uses high-quality chromatin, use of validated ChIP-grade antibodies, and optimized reagents to produce meaningful results.

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